Hormonal blood tests should be done in the morning and on an empty stomach.
Before the test, avoid:
Physical or emotional stress
Physiotherapy procedures
Alcohol and fatty foods
Medication intake, unless otherwise directed by your doctor
For Women:
Unless otherwise instructed by your doctor, the tests should be done on specific days of the menstrual cycle:
LH, FSH, Estradiol: on days 2 to 5
Progesterone: on days 19 to 24
17-OH Progesterone: on days 4 to 5
Prolactin: recommended to be tested 2 hours after waking up
Cortisol: must be tested before 10:00 AM